4.3 3-5分SCI文章
图5来自文献Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes.Stem Cell Res Ther. 2019 Mar 29;10(1):109. 本文分析的是细胞外囊泡(30-1000nm)所以EV有多个峰。EV分离方法为超速离心法。粒径分析仪器:Nanosight NS300.
图6来自文献Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. Int J Oncol. 2019 May;54(5):1719-1733. 采用试剂盒exoEasy Maxi kit,粒径分析仪器:Nanosight NS300.
5. 英文方法写作
范例一
Nanoparticle tracking analysis
To analyse the size distribution of exosomes, NanoSight N300 (Malvern Instruments, Malvern, UK) was used. The samples were monitored with the use of a 640 nm laser. The exosomal sample volume was normalized to equal number (1 × 106) of XX and YY cells. The exosomal aggregations were separated using needle and syringe and simultaneously injected to the NanoSight sample cubicle. The frame rate used was 30 frames per second and NanoSight NTA3.2 software was used for data analysis.
范例二
Nanoparticle tracking analysis
The number and size of the exosomes were directly tracked by the rate of Brownian motion of exosomes using the NanoSight NS 300 system (NanoSight Technology, Malvern, UK), configured with a high-sensitivity sCMOS camera, fast video capture, and particle-tracking software (NanoSight, Amesbury, UK). The samples were diluted 150–3000 times with Dulbecco’s PBS (DPBS) without any nanoparticles to attain a concentration of 1–20 × 108 particles per milliliter for analysis. Each sample was measured in triplicate at the camera, which recorded and tracked each visible particle. Exosome numbers and size distribution were explored using the Stokes-Einstein equation.
范例三
Nanoparticle tracking analysis
Nanoparticle tracking analysis (NTA) was carried out as previously described using the NanoSight system (NanoSight; Wiltshire, UK, www.malvernpanalytical.com/en/) on EVs suspended in PBS. Vesicles were 50 fold diluted in PBS and visualized by light scattering using a conventional optical microscope aligned perpendicularly to the beam axis. NTA software tracked between frames the Brownian motion of inpidual vesicles calculating the total concentration and size through the application of Stokes-Einstein equation.
范例四
Nanoparticle tracking analysis (NTA)
Exosome concentration was analyzed using a NanoSight LM10 system (Nano sight Ltd, Navato, CA) equipped with a blue laser (405 nm). Nanoparticles were illuminated by the laser, and their movement under Brownian motion was captured for 60 seconds. The process was repeated three times. Then, all the three recorded videos were subjected to NTA using the Nanosight particle tracking software (Version NTA 3.1) to calculate exosome concentrations and size distribution.
